Visceral adipose tissue was obtained during biliopancreatic diversion surgery, rapidly frozen in liquid N2 and stored at -80 oC.
Extracted molecule
genomic DNA
Extraction protocol
Genomic DNA was extracted from the VAT using the DNeasy Blood & Tissue kit, as recommended by the manufacturer
Label
biotin
Label protocol
Illumina protocol
Hybridization protocol
Illumina protocol
Scan protocol
Illumina protocol
Data processing
The GenomeStudio software version 2011.1 (Illumina Inc.) and the Methylation Module were used. Normalization method described in: Fortin, J.-P. et al. Functional normalization of 450k methylation array data improves replication in large cancer studies. bioRxiv (2014).