|
| Status |
Public on Jun 09, 2015 |
| Title |
genomic DNA from Brain Frontal of subject 31 |
| Sample type |
genomic |
| |
|
| Source name |
Brain Frontal
|
| Organism |
Homo sapiens |
| Characteristics |
subjectid: 31
tissue: Brain Frontal
Sex: F
sexpredictedusingdnamethdata: female
dna.extraction.method: AllPrep
age: 53
dnamethylationage: 46.58211985
ageaccelerationresidualwithrespecttocontrols: 3.95119760039429
ageaccelerationresidualwithrespecttocontrolsoccipitalonly: NA
postmorteminterval: 12
hivstatus: Control
hiv.viralloadinblood: NA
hivstatusdetectableviralloadinblood: Control
hiv.viralloadincerebrospinalfluid: NA
|
| Treatment protocol |
Standard Illumina Protocol
|
| Growth protocol |
DNA extracted from fresh frozen brain tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using AllPrep DNA/RNA mini kit according to standard instructions
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Sample120
genomic DNA from Brain Frontal of Control subject
|
| Data processing |
BeadStudio software v3.2.
|
| |
|
| Submission date |
Jul 16, 2014 |
| Last update date |
Jun 09, 2015 |
| Contact name |
Steve Horvath |
| E-mail(s) |
shorvath@altoslabs.com
|
| Organization name |
University of California, Los Angeles
|
| Department |
Human Genetics
|
| Lab |
Horvath
|
| Street address |
695 Charles E. Young Drive South, Box 708822
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE59457 |
DNA methylation levels of various brain regions from HIV+ and HIV- subjects |
|
