|
| Status |
Public on May 23, 2014 |
| Title |
genomic DNA from BI treated HepG2_3 |
| Sample type |
genomic |
| |
|
| Source name |
hepatic carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hepatic carcinoma cell
cell line: HepG2
tissue: liver
|
| Treatment protocol |
R-HepG2 cells were treated with 1 ml serum-free DMEM overnight, after which the cell culture medium was replaced with 1mL DMEM or 1 ml BI solution (total insulin concentration was identical, 40 ug/ml). The cells were incubated at 37 °C and 5% CO2 for 4 h and washed using phosphate-buffered saline (PBS, pH = 7.4) and 0.03% ethylene diamine tetraacetic acid (EDTA) to remove any residual complexes.
|
| Growth protocol |
R-HepG2 cells were cultured on tissue culture dishes in DMEM supplemented with 10% FCS, 50 mg/mL penicillin and 50 mg/mL streptomycin
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified using Qiagen DNeasy Kit according to standard instructions
|
| Label |
biotin,DNP
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Infinium HumanMethylation450 BeadArray using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
HepG2 cells
|
| Data processing |
Normalization method by Average Beta
Unmethylated and methylated signal intensities are provided in supplementary file on series level.
|
| |
|
| Submission date |
May 22, 2014 |
| Last update date |
May 23, 2014 |
| Contact name |
Yun Xiao |
| E-mail(s) |
xy91269264@163.com
|
| Organization name |
Zhejiang University
|
| Street address |
Yuhangtang Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE57894 |
Towards epigenetic understanding and therapy of insulin resistance by intranuclear insulin [DNA methylation] |
| GSE57928 |
Towards epigenetic understanding and therapy of insulin resistance by intranuclear insulin |
|
