|
| Status |
Public on May 15, 2014 |
| Title |
Sertoli Cell Line noAR_3 |
| Sample type |
RNA |
| |
|
| Source name |
Sertoli cell line, not expressing AR
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: 93RS2
cell type: Sertoli cell line
androgen receptor expression: no
|
| Treatment protocol |
Cells were not transfected and were harvested after 24h growth after last passage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the "peqGOLD total RNA kit" (#12-6634-01) from PEQLab according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Extracted RNA was subjected to cRNA synthesis and cRNA fragmentation using the CodeLink Expression Assay Kit (GE Healthcare) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was hybridized on the CodeLink Human Whole Genome using the CodeLink Expression Assay Kit (GE Healthcare) according to manufacturer's instructions. Bioarrays were stained with Cy5™-streptavadin (GE Healthcare).
http://www.fda.gov/downloads/scienceresearch/bioinformaticstools/microarrayqualitycontrolproject/ucm132970.pdf
|
| Scan protocol |
GenePix® 4000 B scanner and the GenePix Pro 4.0 Software (Axon Instruments, Arlington, USA).
|
| Description |
Sertoli cells derived from rat testis.
Biological replicate 3 of 3. Sertoli cells lacking any AR (93RS2), harvested after 24h growth after last passage.
|
| Data processing |
Data were median normalized (normalized to median = 1; intra-slide normalization) and local background subtracted using CODELINK Version 5.1.
|
| |
|
| Submission date |
May 14, 2014 |
| Last update date |
May 15, 2014 |
| Contact name |
Melanie Markmann |
| E-mail(s) |
melanie.markmann@chiru.med.uni-giessen.de
|
| Phone |
0049 641 9944492
|
| Organization name |
University of Giessen
|
| Department |
Anaesthesiology
|
| Lab |
Medical Informatic
|
| Street address |
Rudolf-Buchheim-Str. 7
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18683 |
| Series (1) |
| GSE57653 |
Transfection of rat Sertoli cells with human androgen receptor leads to alternated gene expression without androgen incubation |
|
