|
Status |
Public on Apr 25, 2014 |
Title |
N-2 |
Sample type |
RNA |
|
|
Source name |
placenta
|
Organism |
Homo sapiens |
Characteristics |
tissue: villous tissue group: normal
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol was used to extract total RNA.
|
Label |
biotin
|
Label protocol |
Biotinylated template was prepared according to the standard Affymetrix protocol from 1 ug total RNA (FlashTag Biotin HSR RNA Labeling Kit, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 1 ug of labeled product was hybridized for 18 hr at 48° C on GeneChip miRNA 2.0 arrays . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
Data processing |
The data were analyzed with Expression Console 1.2 using RMA + DABG as the normalization method.
|
|
|
Submission date |
Apr 24, 2014 |
Last update date |
Apr 25, 2014 |
Contact name |
Yuping Wang |
E-mail(s) |
ywang1@lsuhsc.edu
|
Phone |
318-675-5379
|
Organization name |
LSUHSC-Shreveport
|
Department |
OB/GYN
|
Street address |
1501 Kings Hwy
|
City |
Shreveport |
State/province |
LA |
ZIP/Postal code |
71103 |
Country |
USA |
|
|
Platform ID |
GPL14613 |
Series (1) |
GSE57050 |
miRNA Profile in Normal and Preeclamptic Placenta |
|