|
| Status |
Public on Apr 25, 2014 |
| Title |
PE-5 |
| Sample type |
RNA |
| |
|
| Source name |
placenta
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: villous tissue
group: preeclamptic
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol was used to extract total RNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated template was prepared according to the standard Affymetrix protocol from 1 ug total RNA (FlashTag Biotin HSR RNA Labeling Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of labeled product was hybridized for 18 hr at 48° C on GeneChip miRNA 2.0 arrays . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed with Expression Console 1.2 using RMA + DABG as the normalization method.
|
| |
|
| Submission date |
Apr 24, 2014 |
| Last update date |
Apr 25, 2014 |
| Contact name |
Yuping Wang |
| E-mail(s) |
ywang1@lsuhsc.edu
|
| Phone |
318-675-5379
|
| Organization name |
LSUHSC-Shreveport
|
| Department |
OB/GYN
|
| Street address |
1501 Kings Hwy
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71103 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE57050 |
miRNA Profile in Normal and Preeclamptic Placenta |
|
