Genomic DNA from fresh frozen lung tumor/normal tissue was extracted using Allprep (Qiagen).
Label
Cy3, Cy5
Label protocol
Standard Illumina labelling protocol.
Hybridization protocol
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 BeadChips using the standard Illumina protocol.
Scan protocol
Arrays were imaged using High Scan SQ using the standard recommended Illumina scanner setting.
Data processing
Signal intensities were obtained from GenomeStudio (Illumina), converted to beta-values, filtered, and normalized to remove biases between Infinium I and II probes
Submission date
Mar 19, 2014
Last update date
Oct 05, 2014
Contact name
Johan Staaf
Organization name
SCIBLU - Swegene Centre for Integrative Biology at Lund University