|
| Status |
Public on Jul 29, 2014 |
| Title |
MSC donor2_1.5 kPa |
| Sample type |
RNA |
| |
|
| Source name |
mesenchymal stromal cells (MSC)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: MSC
substrate: 1.5 kPa PDMS
donor: donor2
|
| Treatment protocol |
MSCs were cultivated for 4 passages in parrallel on TCP, 50 kPa PDMS and 1.5 kPa PDMS.
|
| Growth protocol |
MSCs were cultivated in 10% hPL-Medium at 37°C at humified atmosphere containing 5% carbon dioxide with medium changes twice per week. Passaging was carried out upon 80% confluent growth. MSCs were isolated from lipoaspirates after patient’s written consent using guidelines approved by the Ethic committee of the RWTH Aachen University Medical School (Permit Number EK163/07).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 106 cells using the NucleoSpin RNA Isolation Kit.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned using Affymetrix GeneChip Scanner 3000.
|
| Description |
gene expression data of MSC donor2 cultivated for 4 passages on 1.5 kPa PDMS
|
| Data processing |
CEL files were processed using the Affymetrix Power Tools (APT) Software Package. Normalization was done employing RMA (Irizarry et al., Biostatistics 4, 249-264, 2003).
|
| |
|
| Submission date |
Mar 13, 2014 |
| Last update date |
Jul 29, 2014 |
| Contact name |
Wolfgang Wagner |
| E-mail(s) |
wwagner@ukaachen.de
|
| Phone |
+49 241 8088611
|
| Organization name |
RWTH Aachen University
|
| Department |
Helmholtz Institute for Biomedical Engineering
|
| Lab |
Stem Cell Biology and Cellular Engineering
|
| Street address |
Pauwelsstrasse 20
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10739 |
| Series (2) |
| GSE55867 |
Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells [gene expression profiling] |
| GSE55889 |
Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells |
|
