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Status |
Public on Mar 06, 2014 |
Title |
Knock-out mouse 3 |
Sample type |
RNA |
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Source name |
2 month old Pcyt2+/- mice
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Organism |
Mus musculus |
Characteristics |
genetic background: C57BL6 genotype: Pcyt2+/- tissue: liver
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Treatment protocol |
Liver tissue were collected and snap frozen in liquid N2 , before the RNA extraction.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted after homogeneisation in TRIzol with a tissue homogenizer and using the Qiagen Rneasy kit according to the manufacturer's instructions.
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Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol using 100 ng total RNA (WT Expression Kit User Manual, 2010, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 20 hr at 48C on MoGene 1.1 ST arrays. GeneChips were washed and stained in the Gene Atlas Fluidics Station.
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Scan protocol |
MoGene 1.1 ST arrays were scanned using the Gene Atlas Imaging Station.
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Description |
Gene expression data from 2 month old Pcyt2+/- mice liver
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Data processing |
The data was analyzed using JMP genomics (SAS, Cary, NC) and normalized using the RMA method. An ANOVA was used to identify differentially expressed genes. A FDR of 0.05 was used to account for multiple testing.
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Submission date |
Mar 05, 2014 |
Last update date |
Mar 06, 2014 |
Contact name |
marica bakovic |
E-mail(s) |
pbasu@uoguelph.ca
|
Organization name |
university of Guelph
|
Department |
HHNS
|
Street address |
50 Stone road east
|
City |
Guelph |
State/province |
ON |
ZIP/Postal code |
N1G2W1 |
Country |
Canada |
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|
Platform ID |
GPL11533 |
Series (1) |
GSE55617 |
Gene expression of liver tissue from Pcyt2 mice |
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