|
| Status |
Public on Mar 06, 2014 |
| Title |
Knock-out mouse 2 |
| Sample type |
RNA |
| |
|
| Source name |
2 month old Pcyt2+/- mice
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL6
genotype: Pcyt2+/-
tissue: liver
|
| Treatment protocol |
Liver tissue were collected and snap frozen in liquid N2 , before the RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted after homogeneisation in TRIzol with a tissue homogenizer and using the Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol using 100 ng total RNA (WT Expression Kit User Manual, 2010, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 20 hr at 48C on MoGene 1.1 ST arrays. GeneChips were washed and stained in the Gene Atlas Fluidics Station.
|
| Scan protocol |
MoGene 1.1 ST arrays were scanned using the Gene Atlas Imaging Station.
|
| Description |
Gene expression data from 2 month old Pcyt2+/- mice liver
|
| Data processing |
The data was analyzed using JMP genomics (SAS, Cary, NC) and normalized using the RMA method. An ANOVA was used to identify differentially expressed genes. A FDR of 0.05 was used to account for multiple testing.
|
| |
|
| Submission date |
Mar 05, 2014 |
| Last update date |
Mar 06, 2014 |
| Contact name |
marica bakovic |
| E-mail(s) |
pbasu@uoguelph.ca
|
| Organization name |
university of Guelph
|
| Department |
HHNS
|
| Street address |
50 Stone road east
|
| City |
Guelph |
| State/province |
ON |
| ZIP/Postal code |
N1G2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE55617 |
Gene expression of liver tissue from Pcyt2 mice |
|
