|
Status |
Public on Jan 12, 2015 |
Title |
Silver Russell patient 6 |
Sample type |
genomic |
|
|
Source name |
SRS6
|
Organism |
Homo sapiens |
Characteristics |
gender: female tissue: whole blood disease state: SRS
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from whole blood samples of Silver Russell patients using a standard phenol-chloroform kit (Sigma), and from PBMC samples of healthy controls using a Ficoll-density gradient.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium 450k Human Methylation Beadchip using the standard Illumina protocol.
|
Scan protocol |
Arrays were imaged using an iScan instrument with standard recommended Illumina scanner settings.
|
Description |
Silver Russell patient 6
|
Data processing |
Bioconductor packages minfi v1.6.0 and IlluminaHumanMethylation450kmanifest v0.4.0
|
|
|
Submission date |
Mar 02, 2014 |
Last update date |
Jan 12, 2015 |
Contact name |
Reiner Schulz |
E-mail(s) |
reiner.schulz@kcl.ac.uk
|
Organization name |
King's College London
|
Department |
Medical & Molecular Genetics
|
Lab |
Epigenetics
|
Street address |
8th floor Guy's Tower
|
City |
London |
ZIP/Postal code |
SE1 9RT |
Country |
United Kingdom |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE55491 |
Genomewide methylation analysis in Silver Russell syndrome patients |
|