|
| Status |
Public on Jan 12, 2015 |
| Title |
normal control 8 |
| Sample type |
genomic |
| |
|
| Source name |
PBL8
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: PBMC
disease state: normal
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole blood samples of Silver Russell patients using a standard phenol-chloroform kit (Sigma), and from PBMC samples of healthy controls using a Ficoll-density gradient.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium 450k Human Methylation Beadchip using the standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using an iScan instrument with standard recommended Illumina scanner settings.
|
| Description |
normal control 8
|
| Data processing |
Bioconductor packages minfi v1.6.0 and IlluminaHumanMethylation450kmanifest v0.4.0
|
| |
|
| Submission date |
Mar 02, 2014 |
| Last update date |
Jan 12, 2015 |
| Contact name |
Reiner Schulz |
| E-mail(s) |
reiner.schulz@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Medical & Molecular Genetics
|
| Lab |
Epigenetics
|
| Street address |
8th floor Guy's Tower
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE55491 |
Genomewide methylation analysis in Silver Russell syndrome patients |
|
