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Status |
Public on Jun 09, 2014 |
Title |
Placenta_3.1_SF_M60_Piece_2 |
Sample type |
RNA |
|
|
Source name |
Placenta_3.1_SF_M60_piece
|
Organism |
Homo sapiens |
Characteristics |
tissue id: Placenta 3.1 pregnancy stage: Third trimester tissue collection method: Snap Frozen time after removal from the uterus: 60 minutes tissue type: Piece
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissue was lysed in mirVana (Ambion) lysis buffer, using a Mini-Beadbeater-16 (Biospec), with agitation for one minute in the presence of 1 mm zirconia beads. Samples were then centrifuged at maximum speed for one minute and the lysed solution was transferred to a fresh microfuge tube. The remainder of the extraction was per the manufacturer’s protocol for the mirVana kit (Ambion). After extraction, RNA was quantified using the Quant-iT Kit (Life Technologies). RNA quality was assessed using the Agilent 2100 Bioanalyzer (Agilent).
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
SAMPLE 38
|
Data processing |
The data were filtered for detection pValue ≤ 0.01 and normalised using robust spline normalisation with lumi in R and batch corrected using ComBat.
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|
|
Submission date |
Feb 27, 2014 |
Last update date |
Jun 09, 2014 |
Contact name |
Rathi D Thiagarajan |
E-mail(s) |
rthiagarajan@ucsd.edu
|
Organization name |
UCSD
|
Department |
Reproductive Medicine
|
Lab |
Louise Laurent
|
Street address |
2880 Torrey Pines Scenic Drive
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE55439 |
Banking Placental Tissue: An Optimized Collection Procedure for Genome-Wide Analysis of Nucleic Acids [gene expression] |
GSE55440 |
Banking Placental Tissue: An Optimized Collection Procedure for Genome-Wide Analysis of Nucleic Acids |
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