|
Status |
Public on Feb 25, 2014 |
Title |
Old [F64D41WB] |
Sample type |
RNA |
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|
Source name |
female breast
|
Organism |
Homo sapiens |
Characteristics |
Sex: female age: 64
|
Treatment protocol |
The cells were collected from 10cm2 culture dishes using RLT lysis buffer from the Qiagen miRNeasy kit, were stored at -80°C until RNA extraction.
|
Growth protocol |
The cells were grown in DMEM High Glucose + stable Glutamine (EK 2 mM) (PAA #E15-883) + 1 % v/v AA (von PAA # P11-002 1% v/v) + 10 % FCS GOLD (PAA # A15-151)
|
Extracted molecule |
total RNA |
Extraction protocol |
The RNA was extracted using the provider's recommendations for the Qiagen miRNeasy kit, the RIN was measured, only high quality RNA (RIN 9-10) was used for hybridisation.
|
Label |
Biotin
|
Label protocol |
FlashTag Biotin HSR RNA Labeling procedure as recommended by Affymetrix, as start material 1µg RNA was used.
|
|
|
Hybridization protocol |
Labelled cRNA was hybridized to Affymetrix GeneChip miRNA 2.0 Microarrays for 17h at 48 °C, stained by strepatavidin/phycoerythrin conjugate.
|
Scan protocol |
The chips were scanned as described in the manufacturers´ protocol (FlashTag™ Biotin HSR RNA Labeling Kit P/N 703095 Rev. 2).
|
Description |
miRNA expression in fibroblasts derived from female breast
|
Data processing |
The data were normalized using Robust Multiarray Average (RMA) within the R environment for statistical computing. Non-human miRNAs and miRNAs with intensity below 80 in all age groups were excluded. The GSE55299.txt contains only human miRNAs that passed the expression threshold.
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|
|
Submission date |
Feb 24, 2014 |
Last update date |
Feb 25, 2014 |
Contact name |
Steffen Sass |
E-mail(s) |
steffen.sass@helmholtz-muenchen.de
|
Organization name |
Helmholtz Zentrum München
|
Street address |
Ingolstädter Landstr. 1
|
City |
Neuherberg |
ZIP/Postal code |
85764 |
Country |
Germany |
|
|
Platform ID |
GPL14613 |
Series (1) |
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