|
| Status |
Public on Feb 25, 2014 |
| Title |
Young [F26D29WB] |
| Sample type |
RNA |
| |
|
| Source name |
female breast
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
age: 26
|
| Treatment protocol |
The cells were collected from 10cm2 culture dishes using RLT lysis buffer from the Qiagen miRNeasy kit, were stored at -80°C until RNA extraction.
|
| Growth protocol |
The cells were grown in DMEM High Glucose + stable Glutamine (EK 2 mM) (PAA #E15-883) + 1 % v/v AA (von PAA # P11-002 1% v/v) + 10 % FCS GOLD (PAA # A15-151)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted using the provider's recommendations for the Qiagen miRNeasy kit, the RIN was measured, only high quality RNA (RIN 9-10) was used for hybridisation.
|
| Label |
Biotin
|
| Label protocol |
FlashTag Biotin HSR RNA Labeling procedure as recommended by Affymetrix, as start material 1µg RNA was used.
|
| |
|
| Hybridization protocol |
Labelled cRNA was hybridized to Affymetrix GeneChip miRNA 2.0 Microarrays for 17h at 48 °C, stained by strepatavidin/phycoerythrin conjugate.
|
| Scan protocol |
The chips were scanned as described in the manufacturers´ protocol (FlashTag™ Biotin HSR RNA Labeling Kit P/N 703095 Rev. 2).
|
| Description |
miRNA expression in fibroblasts derived from female breast
|
| Data processing |
The data were normalized using Robust Multiarray Average (RMA) within the R environment for statistical computing. Non-human miRNAs and miRNAs with intensity below 80 in all age groups were excluded. The GSE55299.txt contains only human miRNAs that passed the expression threshold.
|
| |
|
| Submission date |
Feb 24, 2014 |
| Last update date |
Feb 25, 2014 |
| Contact name |
Steffen Sass |
| E-mail(s) |
steffen.sass@helmholtz-muenchen.de
|
| Organization name |
Helmholtz Zentrum München
|
| Street address |
Ingolstädter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
|
