|
| Status |
Public on Feb 20, 2014 |
| Title |
patient 33, ccRCC tissue |
| Sample type |
RNA |
| |
|
| Source name |
ccRCC tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Following excision, the tissue samples were immediately immersed in RNAlater, (QIAGEN) and stored at −20°C until RNA extraction. miRNAs were purified using the miRNeasy mini kit (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Total RNA 100 ng were subjected to cDNA synthesis by Ambion WT Expression Kit (Life technologies). Obtained 5.5 μg cDNA were biotinylated and then fragmented by GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
After creating the hybridization cocktail, biotinylated cDNA were hybridized with GeneChip Human Exon 1.0 ST Array (Affymetrix) for 17 hours.
|
| Scan protocol |
The arrays were scanned using the Affymetrix Gene Chip Scanner 3000.
|
| Description |
miRNA expression data from ccRCC tissue
|
| Data processing |
Scanned data were processed using the Agilent GeneSpring GX software.
|
| |
|
| Submission date |
Feb 19, 2014 |
| Last update date |
Feb 20, 2014 |
| Contact name |
Kentaro Jingushi |
| E-mail(s) |
jingushi-kk@phs.osaka-u.ac.jp
|
| Phone |
+81-6-6879-8192
|
| Organization name |
Osaka University
|
| Department |
Graduate School of Pharmaceutical Sciences
|
| Lab |
Laboratory of Molecular and Cellular Physiology
|
| Street address |
1-6 Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE55138 |
Expression data from ccRCC specimens |
|
