|
| Status |
Public on Sep 15, 2014 |
| Title |
genomic DNA_sporadic melanoma 5 |
| Sample type |
genomic |
| |
|
| Source name |
sporadic melanoma 5
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cdkn2a status: wild type
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from leukocytes using Gentra Puregene Blood Core Kit (Qiagen) or QIAmp DNA Blood Mini QIACUBE kit (Quiagen)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA (EZ DNA methylation kit; Zymo Research) was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using Illumina iScan according standard recommended Illumina scanner setting
|
| Description |
leukocyte's DNA from sporadic melanoma patient 5
|
| Data processing |
Data normalization was performed using the pipeline described in Touleimat and Tost, 2012 (Epigenomics, 4(3):325-41)
|
| |
|
| Submission date |
Feb 12, 2014 |
| Last update date |
Sep 15, 2014 |
| Contact name |
Érica Sara Souza de Araújo |
| E-mail(s) |
erica.sara.araujo@gmail.com
|
| Organization name |
A. C. Camargo Cancer Center
|
| Department |
CIPE - Internacional Center for Research and Training
|
| Street address |
Taguá st, 440
|
| City |
Sao Paulo |
| State/province |
Sao Paulo |
| ZIP/Postal code |
01508010 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE54939 |
Leukocytes methylome of melanoma patients with and without CDKN2A mutations |
|
