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Status |
Public on Feb 28, 2014 |
Title |
MD_MB_155 |
Sample type |
genomic |
|
|
Source name |
Primary medulloblastoma tumor tissue
|
Organism |
Homo sapiens |
Characteristics |
gender: M age (yrs): 4.7 tissue: Primary medulloblastoma tumor tissue molecular subgroup: Group 3 sample type: formalin-fixed paraffin-embedded (FFPE)
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Extraction of high molecular weight DNA from frozen tumor samples was carried out using a Qiagen Allprep DNA/RNA/Protein Mini Kit.
|
Label |
biotin/DNP
|
Label protocol |
According to the manufacturer’s instructions
|
|
|
Hybridization protocol |
According to the manufacturer’s instructions
|
Scan protocol |
Illumina iScan System, according to the manufacturer’s instructions
|
Description |
MD_MB_155.AVG_Beta
|
Data processing |
Array processing was performed using Illumina GenomeStudio V2011.1 (Methylation Module version 1.9.0). The 'MB-450k_GEOupload_Raw.txt' contains unmethylated and methylated signal intensities. Signal intensities were obtained without background subtraction and normalized to internal controls. The 'MB-450k_GEOupload_Beta.txt' contains 'Beta-values'.
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|
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Submission date |
Feb 11, 2014 |
Last update date |
Feb 28, 2014 |
Contact name |
Peter Lichter |
Organization name |
German Cancer Research Center
|
Department |
Molecular Genetics
|
Street address |
Im Neuenheimer Feld 580
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE54880 |
Microarray-based DNA methylation profiling of medulloblastoma and normal cerebellum samples |
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