|
| Status |
Public on Feb 04, 2014 |
| Title |
genomic DNA from Normal Reconstruction mixture Reconstruction mixture |
| Sample type |
genomic |
| |
|
| Source name |
Normal Reconstruction mixture Reconstruction mixture NormMix
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Reconstruction mixture
disease state: normal
sample subtype: Normal Reconstruction mixture
|
| Treatment protocol |
Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec; venous whole blood was collected into Heparin
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
X8942297054_R01C01
Infinium Type I and Type II Probes
Normal Reconstruction mixture Reconstruction mixture NormMix
|
| Data processing |
Unmethylated and methylated signal intensities generated using methylumi in R (see supplementary file on the Series record).
Average Betas (not normalized) generated using GenomeStudio v2010.3
|
| |
|
| Submission date |
Feb 04, 2014 |
| Last update date |
Feb 04, 2014 |
| Contact name |
William P Accomando |
| Organization name |
Harvard School of Public Health
|
| Department |
Epidemiology
|
| Street address |
Kresge Bldg 911, 677 Huntington Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE54670 |
Illumina Infinium HumanMethylation450 data for human whole blood samples and for mixtures of purified white blood cells reconstructing human blood |
| GSE54671 |
Quantitative reconstruction of leukocyte subsets using DNA methylation |
|
