|
| Status |
Public on Jan 16, 2014 |
| Title |
genomic DNA from cultured cells_iPS OSLN5 |
| Sample type |
genomic |
| |
|
| Source name |
iPS cells derived using Thompson factors (OSLN)
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: normal
cell type: Induced pluripotent stem (iPS) cells
reprogramming factors used: Thomson factors (OCT4, SOX2, NANOG and LIN28)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from cultured cells using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
induced pluripotent stem cell by OSLN cocktail
|
| Data processing |
BeadStudio software; the non_normalized.txt contains unmethylated and methylated signal intensities.
|
| |
|
| Submission date |
Jan 15, 2014 |
| Last update date |
Jan 16, 2014 |
| Contact name |
Daniel De Carvalho |
| E-mail(s) |
ddecarv@uhnresearch.ca
|
| Organization name |
Ontario Cancer Institute
|
| Street address |
101 College Street, room 9-302
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE54115 |
Aberrant DNA methylation reprogramming during iPS cell generation is dependent on the choice of reprogramming factors |
|
