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Status |
Public on Jan 16, 2014 |
Title |
genomic DNA from cultured cells_iPS OSLN3 |
Sample type |
genomic |
|
|
Source name |
iPS cells derived using Thompson factors (OSLN)
|
Organism |
Homo sapiens |
Characteristics |
disease state: normal cell type: Induced pluripotent stem (iPS) cells reprogramming factors used: Thomson factors (OCT4, SOX2, NANOG and LIN28)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from cultured cells using Qiagen DNeasy Kit according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
induced pluripotent stem cell by OSLN cocktail
|
Data processing |
BeadStudio software; the non_normalized.txt contains unmethylated and methylated signal intensities.
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|
|
Submission date |
Jan 15, 2014 |
Last update date |
Jan 16, 2014 |
Contact name |
Daniel De Carvalho |
E-mail(s) |
ddecarv@uhnresearch.ca
|
Organization name |
Ontario Cancer Institute
|
Street address |
101 College Street, room 9-302
|
City |
Toronto |
State/province |
ON |
ZIP/Postal code |
M5G 1L7 |
Country |
Canada |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE54115 |
Aberrant DNA methylation reprogramming during iPS cell generation is dependent on the choice of reprogramming factors |
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