|
Status |
Public on Dec 13, 2013 |
Title |
WG1282 [gene expression] |
Sample type |
genomic |
|
|
Source name |
WG1282
|
Organism |
Homo sapiens |
Characteristics |
cell line: WG1282 gender: male cell type: primary skin fibroblast sample
|
Treatment protocol |
NA
|
Growth protocol |
Cells were grown in alpha MEM Medium (SigmaAldrich, Oakville, ON, Canada) supplemented with 2 mmol/l L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% fetal bovine serum (SigmaAldrich) at 37°C with 5% CO2 to 70-80% confluence, then harvested and stored at -80°C until RNA and DNA was extracted.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using a standard protocol involving TRIzol and phenol-chloroform extraction.
|
Label |
Cy3
|
Label protocol |
standard Illumina protocol
|
|
|
Hybridization protocol |
standard Illumina protocol
|
Scan protocol |
arrays were imaged using iScan Reader using standard recommended Illumina scanner setting
|
Data processing |
Data were log2 transformed and quantile normalized with the lumi package in R
|
|
|
Submission date |
Dec 12, 2013 |
Last update date |
Dec 13, 2013 |
Contact name |
Stephan Busche |
Organization name |
McGill University
|
Department |
Human Genetics
|
Street address |
740 Dr. Penfield Avenue
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3A 0G1 |
Country |
Canada |
|
|
Platform ID |
GPL6883 |
Series (2) |
GSE53243 |
The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts [expression profiling] |
GSE53261 |
The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts |
|