|
| Status |
Public on Jan 01, 2016 |
| Title |
diseased (E_BC_CA_027_5935534023.RO3C02) |
| Sample type |
genomic |
| |
|
| Source name |
62 buffy coat
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: diseased (case, breast cancer)
tissue: blood
tissue fraction: buffy coat
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from LBC samples using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450K Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
genomic DNA from buffy coat
|
| Data processing |
BeadStudio software v3.2 and a novel intensity-based normalization method using technical quality controls (see ralated publication)
|
| |
|
| Submission date |
Nov 21, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
panagiotis georgiadis |
| E-mail(s) |
panosg@eie.gr
|
| Phone |
302107273743
|
| Organization name |
NHRF
|
| Department |
IBMCB
|
| Lab |
Chemical Carcinogenesis
|
| Street address |
48 vas costantinou
|
| City |
athens |
| State/province |
Greece |
| ZIP/Postal code |
11635 |
| Country |
Greece |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE52635 |
A Composite Framework for the Statistical Analysis of Epidemiological DNA Methylation Data with the Infinium Human Methylation 450K BeadChip |
|
