|
| Status |
Public on Dec 11, 2013 |
| Title |
GM00022_cDNA |
| Sample type |
RNA |
| |
|
| Source name |
GM00022
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell type: primary skin fibroblast
population: Caucasian
|
| Biomaterial provider |
http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?Ref=GM00022
|
| Growth protocol |
The Fibroblast primary cell lines were grown in medium containing a-MEM (SigmaAldrich) supplemented with 2 mmol/l L-Glutamine, 100 U/mL penicillin, 100 mg/ml streptomycin, and 10% fetal bovine serum (SigmaAldrich) at 37°C with 5% CO2. At 70%-80% confluence, the cells were harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from cell lysates resuspended in 600 mL RLT lysis buffer using the Rneasy Mini Kit (Qiagen). DNA was extracted from cell lysates resuspended in 200 mL PBS using the GenElute DNA Miniprep Kit (SigmaAldrich).
|
| Label |
Cy3 and Cy5
|
| Label protocol |
according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
according to manufacturer's instructions
|
| Scan protocol |
according to manufacturer's instructions
|
| Description |
Family 491 , mother
|
| Data processing |
Using GenomeStudio from Illumina
|
| |
|
| Submission date |
Nov 16, 2013 |
| Last update date |
Dec 11, 2013 |
| Contact name |
Tomi Markku Pastinen |
| E-mail(s) |
Tomi.Pastinen@mcgill.ca
|
| Organization name |
McGill University and Génome Québec Innovation Centre
|
| Street address |
740, Dr. Penfield Avenue
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 1A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6984 |
| Series (2) |
| GSE52442 |
The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts [genotyping] |
| GSE53261 |
The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts |
|
