|
| Status |
Public on Sep 25, 2014 |
| Title |
MSC cultured in human MSC media, MSC-20 |
| Sample type |
genomic |
| |
|
| Source name |
human mesenchymal stem cells, 77-year-old female
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: mesenchymal stem cells (MSCs)
gender: female
age: 77 years
disease state: healthy
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was isolated by standard procedures
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol.
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.
|
| Description |
Healthy sample
|
| Data processing |
Subset-quantile Within Array Normalization (SWAN) using the R-Bioconductor/minfi package.
|
| |
|
| Submission date |
Nov 05, 2013 |
| Last update date |
Sep 25, 2014 |
| Contact name |
Agustin F Fernandez |
| E-mail(s) |
affernandez@hca.es, agusff@gmail.com
|
| Phone |
985652411
|
| Organization name |
Oviedo University-HUCA
|
| Department |
IUOPA
|
| Lab |
Epigenetics
|
| Street address |
Avenida de Roma s/n
|
| City |
Oviedo |
| State/province |
Asturias |
| ZIP/Postal code |
33011 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE52112 |
H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells [MSCs] |
| GSE52114 |
H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells |
|
