|
Status |
Public on Sep 25, 2014 |
Title |
MSC cultured in human MSC media, MSC-5 |
Sample type |
genomic |
|
|
Source name |
human mesenchymal stem cells, 10-year-old female
|
Organism |
Homo sapiens |
Characteristics |
cell type: mesenchymal stem cells (MSCs) gender: female age: 10 years disease state: healthy
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Total DNA was isolated by standard procedures
|
Label |
Cy3, Cy5
|
Label protocol |
Standard Illumina labelling protocol.
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol.
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.
|
Description |
Healthy sample
|
Data processing |
Subset-quantile Within Array Normalization (SWAN) using the R-Bioconductor/minfi package.
|
|
|
Submission date |
Nov 05, 2013 |
Last update date |
Sep 25, 2014 |
Contact name |
Agustin F Fernandez |
E-mail(s) |
affernandez@hca.es, agusff@gmail.com
|
Phone |
985652411
|
Organization name |
Oviedo University-HUCA
|
Department |
IUOPA
|
Lab |
Epigenetics
|
Street address |
Avenida de Roma s/n
|
City |
Oviedo |
State/province |
Asturias |
ZIP/Postal code |
33011 |
Country |
Spain |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE52112 |
H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells [MSCs] |
GSE52114 |
H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells |
|