|
| Status |
Public on Dec 10, 2013 |
| Title |
WG1978 |
| Sample type |
genomic |
| |
|
| Source name |
genomic DNA from WG1978
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: skin
cell type: fibroblast
cell line: WG1978
|
| Treatment protocol |
NA
|
| Growth protocol |
Cells were grown in alpha MEM Medium (SigmaAldrich, Oakville, ON, Canada) supplemented with 2 mmol/l L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% fetal bovine serum (SigmaAldrich) at 37°C with 5% CO2 to 70-80% confluence, then harvested and stored at -80°C until RNA and DNA was extracted.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from cell pellets using the DNeasy Blood and Tissue Kit (Qiagen) according to manufacturer’s protocol.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
arrays were imaged using iScan Reader using standard recommended Illumina scanner setting
|
| Description |
SAMPLE 46
primary skin fibroblast sample
|
| Data processing |
GenomeStudio Software V2011.1
|
| |
|
| Submission date |
Nov 02, 2013 |
| Last update date |
Nov 04, 2014 |
| Contact name |
Stephan Busche |
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Street address |
740 Dr. Penfield Avenue
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 0G1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE52025 |
The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts [methylation profiling] |
| GSE53261 |
The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts |
|
