|
Status |
Public on Mar 23, 2015 |
Title |
genomic DNA from old sun protected dermis 10 |
Sample type |
genomic |
|
|
Source name |
old sun protected dermis
|
Organism |
Homo sapiens |
Characteristics |
gender: female age: 65 exposure: sun protected tissue: dermis
|
Treatment protocol |
Overnight incubation in dispase at 4 degrees C followed by seperation of epidermal and dermal layers.
|
Growth protocol |
NA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was purified using EpiCentre MasterPure kit according to manufactuerer's instructions.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using Illumina iScan using standard recommended Illumina scanner setting
|
Description |
7512578021_R04C01
|
Data processing |
Minfi Package
|
|
|
Submission date |
Oct 31, 2013 |
Last update date |
Mar 23, 2015 |
Contact name |
Andrew Feinberg |
Organization name |
Johns Hopkins University School of Medicine
|
Street address |
855 N. Wolfe St
|
City |
Baltimore |
State/province |
Maryland |
ZIP/Postal code |
21205 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE51954 |
Epigenome analysis of human epidermal and dermal samples with aging and sun exposure |
GSE52980 |
Epigenome analysis of human epidermal and dermal samples with aging and sun exposure. |
|