|
| Status |
Public on Mar 23, 2015 |
| Title |
genomic DNA from young sun protected dermis 5 |
| Sample type |
genomic |
| |
|
| Source name |
young sun protected dermis
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 27
exposure: sun protected
tissue: dermis
|
| Treatment protocol |
Overnight incubation in dispase at 4 degrees C followed by seperation of epidermal and dermal layers.
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was purified using EpiCentre MasterPure kit according to manufactuerer's instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using Illumina iScan using standard recommended Illumina scanner setting
|
| Description |
7512578021_R01C02
|
| Data processing |
Minfi Package
|
| |
|
| Submission date |
Oct 31, 2013 |
| Last update date |
Mar 23, 2015 |
| Contact name |
Andrew Feinberg |
| Organization name |
Johns Hopkins University School of Medicine
|
| Street address |
855 N. Wolfe St
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE51954 |
Epigenome analysis of human epidermal and dermal samples with aging and sun exposure |
| GSE52980 |
Epigenome analysis of human epidermal and dermal samples with aging and sun exposure. |
|
