|
| Status |
Public on Dec 24, 2013 |
| Title |
npr1-1 SA, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Two week old npr1-1 seedlings, SA treated
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
age: Two weeks old
genotype/variation: npr1-1 mutant
agent: salicylic acid
tissue: complete seedling
|
| Treatment protocol |
After two weeks of growth, the medium was exchanged with either water or 2mM SA and 24h incubation was given in light at 22±2ºC.
|
| Growth protocol |
Seed sterilization and plant growth conditions were followed as reported earlier (Hardtke and Deng, 2000) for both Arabidopsis thaliana Col-0 and npr1-1 mutant. All seeds were grown in vitro in MS medium by keeping them on a mesh at 4°C for 2 days for stratification before placing them in the growth chamber. A. thaliana plants were grown under controlled environmental conditions (22±1ºC, 120 mol m-2 sec-1, 16h light/8h dark cycle).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from complete seedlings using Sigma Spectrum® Plant total RNA kit (STRN 250)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol using 250 ng total RNA.
|
| |
|
| Hybridization protocol |
According to affymatrix gene chip instruction manual using Fluidisc station 450.
|
| Scan protocol |
GeneChips were scanned using Affymatrix gene chip scanner.
|
| Description |
Two week old npr1-1 seedlings, SA treated
|
| Data processing |
affymetrix-algorithm-name = rma
program-name = Expression Console
|
| |
|
| Submission date |
Oct 23, 2013 |
| Last update date |
Dec 24, 2013 |
| Contact name |
Samir Sawant |
| E-mail(s) |
samirsawant@nbri.res.in
|
| Organization name |
National Botanical Research Institute
|
| Department |
Centre of Plant Molecular Biology
|
| Lab |
Plant Molecular Biology and Genetic Engeneering Division
|
| Street address |
Rana Pratap Marg
|
| City |
Lucknow |
| State/province |
U.P, India |
| ZIP/Postal code |
226001 |
| Country |
India |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE51626 |
Role of NPR1 in SA mediated transcription |
|
