|
Status |
Public on Feb 16, 2014 |
Title |
genomic DNA from control 8 |
Sample type |
genomic |
|
|
Source name |
peripheral blood
|
Organism |
Homo sapiens |
Characteristics |
tissue: peripheral blood gender: male phenotype: fertile control
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
genomic DNA |
Extraction protocol |
NucleoSpin 96 Blood kit (Macherey-Nagel, Düren, Germany) and Gentra Puregene Blood Kit (Qiagen, Hilden, Germany)
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were scanned using the Illumina iScan
|
Description |
conceived at least one child
|
Data processing |
GenomeStudio software (version 2011.1, Methylation module version 1.9.0)
|
|
|
Submission date |
Sep 27, 2013 |
Last update date |
Feb 16, 2014 |
Contact name |
Susanne Bens |
E-mail(s) |
sbens@medgen.uni-kiel.de
|
Organization name |
Christian-Albrechts-University Kiel & University Hospital Schleswig-Holstein
|
Department |
Institute of Human Genetics
|
Street address |
Arnold-Heller-Str.3 (Haus 10)
|
City |
Kiel |
ZIP/Postal code |
D-24105 |
Country |
Germany |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE51245 |
Array-based DNA methylation profiling in male infertility reveals allele-specific DNA methylation in PIWIL1 and PIWIL2 |
|