|
| Status |
Public on Feb 16, 2014 |
| Title |
genomic DNA from patient 6 |
| Sample type |
genomic |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
gender: male
phenotype: infertile
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
NucleoSpin 96 Blood kit (Macherey-Nagel, Düren, Germany) and Gentra Puregene Blood Kit (Qiagen, Hilden, Germany)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were scanned using the Illumina iScan
|
| Description |
normal CFTR and AZF testing, normal karyotype
|
| Data processing |
GenomeStudio software (version 2011.1, Methylation module version 1.9.0)
|
| |
|
| Submission date |
Sep 27, 2013 |
| Last update date |
Feb 16, 2014 |
| Contact name |
Susanne Bens |
| E-mail(s) |
sbens@medgen.uni-kiel.de
|
| Organization name |
Christian-Albrechts-University Kiel & University Hospital Schleswig-Holstein
|
| Department |
Institute of Human Genetics
|
| Street address |
Arnold-Heller-Str.3 (Haus 10)
|
| City |
Kiel |
| ZIP/Postal code |
D-24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE51245 |
Array-based DNA methylation profiling in male infertility reveals allele-specific DNA methylation in PIWIL1 and PIWIL2 |
|
