|
| Status |
Public on May 27, 2014 |
| Title |
genomic DNA from brain tumor 241 |
| Sample type |
genomic |
| |
|
| Source name |
brain tumor 31
|
| Organism |
Homo sapiens |
| Characteristics |
gender: F
age: 23
tumor location: Brainstem
idh1/h3f3a mutation status: H3F3A MUT
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted usingWizard® Genomic DNA Purification Kit (Cat.No.A1125, Promega, USA). Extracted DNA was bisulfite treated with the Zymo bisulfite kit according the manufacturer's specifications
|
| Label |
biotin nucleotide or dinitrophenyl labeled
|
| Label protocol |
A single nucleotide extension was labeled either with biotin or dinitrophenyl according to the manufacturer's (Illumina) specifications
|
| |
|
| Hybridization protocol |
Followed the manufacturer's (Illumina) specifications
|
| Scan protocol |
Followed the manufacturer's (Illumina) specifications on an iScan
|
| Description |
SAMPLE 31
|
| Data processing |
Data were interpreted in GenomeStudio v2011.1 Methylation Module 1.9.0 and exported for analysis
matrix signal intensities value definition: Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Sep 11, 2013 |
| Last update date |
May 27, 2014 |
| Contact name |
Hai Yan |
| E-mail(s) |
hai.yan@dm.duke.edu
|
| Organization name |
Duke University
|
| Department |
Pathology
|
| Lab |
Yan Lab
|
| Street address |
199B-MSRB, Research Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE50774 |
DNA methylation alteration and gene expression sigature in brainstem, thalamic, and supratentorial gliomas |
|
