|
Status |
Public on May 27, 2014 |
Title |
genomic DNA from brain tumor YD-67 |
Sample type |
genomic |
|
|
Source name |
brain tumor 25
|
Organism |
Homo sapiens |
Characteristics |
gender: M age: 53 tumor location: Supratentorial idh1/h3f3a mutation status: IDH1 MUT
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted usingWizard® Genomic DNA Purification Kit (Cat.No.A1125, Promega, USA). Extracted DNA was bisulfite treated with the Zymo bisulfite kit according the manufacturer's specifications
|
Label |
biotin nucleotide or dinitrophenyl labeled
|
Label protocol |
A single nucleotide extension was labeled either with biotin or dinitrophenyl according to the manufacturer's (Illumina) specifications
|
|
|
Hybridization protocol |
Followed the manufacturer's (Illumina) specifications
|
Scan protocol |
Followed the manufacturer's (Illumina) specifications on an iScan
|
Description |
SAMPLE 25
|
Data processing |
Data were interpreted in GenomeStudio v2011.1 Methylation Module 1.9.0 and exported for analysis matrix signal intensities value definition: Unmethylated and methylated signal intensities
|
|
|
Submission date |
Sep 11, 2013 |
Last update date |
May 27, 2014 |
Contact name |
Hai Yan |
E-mail(s) |
hai.yan@dm.duke.edu
|
Organization name |
Duke University
|
Department |
Pathology
|
Lab |
Yan Lab
|
Street address |
199B-MSRB, Research Drive
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27710 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE50774 |
DNA methylation alteration and gene expression sigature in brainstem, thalamic, and supratentorial gliomas |
|