|
| Status |
Public on Nov 25, 2014 |
| Title |
Peripheral blood from CXMA-04-414 |
| Sample type |
genomic |
| |
|
| Source name |
Peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
smoking (0, 1 and 2, which represent never, former and current smokers): 1
age: 49
gender: Male
tissue: blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole blood samples using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was hybridised to Illumina Infinium HumanMethylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
CXMA-04-414
|
| Data processing |
GenomeStudio (2010.3) Methylation module (1.8.5)
|
| |
|
| Submission date |
Sep 06, 2013 |
| Last update date |
Nov 25, 2014 |
| Contact name |
Tsun-Po Yang |
| Organization name |
Wellcome Trust Sanger Institute
|
| Department |
Human Genetics
|
| Lab |
Genetics of Complex Traits in Humans
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Cambridge |
| ZIP/Postal code |
CB10 1SA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE50660 |
Cigarette Smoking Reduces DNA Methylation Levels at Multiple Genomic Loci but the Effect is Partially Reversible upon Cessation |
|
