|
Status |
Public on Dec 03, 2013 |
Title |
O22 |
Sample type |
genomic |
|
|
Source name |
vastus lateralis muscle, non-exercised
|
Organism |
Homo sapiens |
Characteristics |
gender: male age: 69 bmi: 26.91426421805
|
Treatment protocol |
none
|
Growth protocol |
Skeletal muscle biopsies from the vastus lateralis
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from muscle samples in a randomized fashion with regards to age using a Qiagen Qiacube DNA extraction robot
|
Label |
Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Standard Illumina Protocol
|
Scan protocol |
Standard Illumina Protocol
|
Description |
old
|
Data processing |
We used the lumi/methylumi packages (Bioconductor) to derive the quantile normalized values of methylation (ß values). To derive sites of potential differential methylation (DM), we compared old versus young ß values using a modified t-statistic, where defined DM sites based on statistical significance controlling false discovery rate
|
|
|
Submission date |
Aug 30, 2013 |
Last update date |
Dec 03, 2013 |
Contact name |
Simon Melov |
Organization name |
Buck Institute for Research on Aging
|
Lab |
Melov Lab
|
Street address |
8001 Redwood Blvd
|
City |
Novato |
State/province |
CA |
ZIP/Postal code |
94945 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE50498 |
Genome wide DNA methylation changes with age in disease free human skeletal muscle |
|