|
| Status |
Public on Dec 03, 2013 |
| Title |
O12 |
| Sample type |
genomic |
| |
|
| Source name |
vastus lateralis muscle, non-exercised
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 71
bmi: 25.0122448979592
|
| Treatment protocol |
none
|
| Growth protocol |
Skeletal muscle biopsies from the vastus lateralis
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from muscle samples in a randomized fashion with regards to age using a Qiagen Qiacube DNA extraction robot
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol
|
| Scan protocol |
Standard Illumina Protocol
|
| Description |
old
|
| Data processing |
We used the lumi/methylumi packages (Bioconductor) to derive the quantile normalized values of methylation (ß values). To derive sites of potential differential methylation (DM), we compared old versus young ß values using a modified t-statistic, where defined DM sites based on statistical significance controlling false discovery rate
|
| |
|
| Submission date |
Aug 30, 2013 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Simon Melov |
| Organization name |
Buck Institute for Research on Aging
|
| Lab |
Melov Lab
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE50498 |
Genome wide DNA methylation changes with age in disease free human skeletal muscle |
|
