|
| Status |
Public on Dec 31, 2014 |
| Title |
iMSC#3, Day 28 osteogenic differentiation (June) |
| Sample type |
genomic |
| |
|
| Source name |
iMSC#3 , Day 28 osteogenic differentiaiton (June)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: iMSC#3
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
treatment: osteogenic differentiaiton
|
| Growth protocol |
The iMSC3 cells were culture in minimum essential medium alpha (MEM alpha) (Invitrogen) supplemented with 10% fetal calf serum (FCS) and glutamax (growth mediun; GM). For osteogenic differentiation GM was supplemented with 67 µM ascorbic acid 2-phosphate (Sigma-Aldrich), 3.5 mM β-glycerol phosphate (Sigma-Aldrich) and 10 nM dexamethasone (Sigma-Aldrich) (differentiation medium; DM) and cells were cultured for 28 days.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega, Madison, USA).
|
| Label |
Biotin and dinitrophenyl
|
| Label protocol |
Standard IIlumina label protocol
|
| |
|
| Hybridization protocol |
Standard IIlumina hyb protocol
|
| Scan protocol |
Standard IIlumina scan protocol
|
| Data processing |
Data extraction and initial quality control of the bead summary raw data were performed using GenomeStudio v2011.1 and the Methylation module v1.9, both provided by Illumina.
|
| |
|
| Submission date |
Aug 06, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
L.A.Meza-Zepeda@ous-research.no
|
| Phone |
+47-99035706
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Tumor Biology
|
| Lab |
Meza-Zepeda
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE49585 |
The regulatory landscape of osteogenic differentiation |
|
