|
| Status |
Public on Aug 30, 2013 |
| Title |
NAFLD liver biopsy tissue 15568 |
| Sample type |
genomic |
| |
|
| Source name |
Liver, percutaneous biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Non-Alcoholic Fatty Liver Disease (NAFLD)
tissue: Liver
Stage: advanced
|
| Treatment protocol |
NA
|
| Growth protocol |
Human liver tissues from percutaneous biopsies were stored at -80 degrees celsius prior to DNA extraction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from frozen liver biopsies using the AllPrep Micro Kit (Qiagen) and 800 ng bisulfite modified using the Zymo EZ DNA Methylation Kit
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Methylation data from NAFLD patient
|
| Data processing |
quantile normalization
|
| |
|
| Submission date |
Aug 05, 2013 |
| Last update date |
Feb 02, 2016 |
| Contact name |
Cynthia Moylan |
| E-mail(s) |
cynthia.moylan@duke.edu
|
| Organization name |
Duke University Medical Center
|
| Department |
Gastroenterology
|
| Street address |
595 LaSalle Street, GSRB1, DUMC 3256
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE31803 |
Methylome-Transcriptome Relationships in Nonalcoholic Fatty Liver Disease |
| GSE49542 |
Methylation profiles in non-alcoholic fatty liver disease |
|
