|
Status |
Public on Sep 08, 2014 |
Title |
Genomic DNA from adenoma patient 18 |
Sample type |
genomic |
|
|
Source name |
colorectal mucosa
|
Organism |
Homo sapiens |
Characteristics |
tissue: colorectal mucosa colon region: Left disease status: adenoma gender: Female
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from fresh-frozen samples using Qiagen DNeasy Kit according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
adenoma
|
Data processing |
Bio-conductor. Use SWAN for data normalization and Combat for batch-effect corrections.
|
|
|
Submission date |
Jul 10, 2013 |
Last update date |
Sep 08, 2014 |
Contact name |
Yanxin Luo |
E-mail(s) |
yluo@fhcrc.org
|
Phone |
206-667-1823
|
Organization name |
Fred Hutchinson Cancer Research Center
|
Department |
Clinical Research Division
|
Lab |
D4-255
|
Street address |
1100 Fairview Ave N
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE48684 |
Global DNA methylation alterations reveal multiple pathways in the initiation and progression of colorectal cancer |
|