|
Status |
Public on Sep 12, 2014 |
Title |
Normal tissue_GR37 |
Sample type |
RNA |
|
|
Source name |
Breast normal tissue
|
Organism |
Homo sapiens |
Characteristics |
subtype: - age: 40
|
Treatment protocol |
All samples did not receive neoadjuvant treatment
|
Growth protocol |
All samples were locked in parafine-formaline (FFPE) and stored at room temperature
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted from four 20μm-thick sections of FFPE tissue samples using RecoverAll Total Nucleic Acid Isolation Kit (Applied Biosystems, Life Technologies)
|
Label |
biotin
|
Label protocol |
300ng of total RNA was labeled with Hy3 fluorescent dye
|
|
|
Hybridization protocol |
RNA from each sample was hybridized over 18h at 56ºC onto a GeneChip miRNA 2.0 array
|
Scan protocol |
Chips were scanned using an Agilent scanner, with the scan laser set to 635nm, scan resolution of 10μm, power 80 and PMT 70.
|
Data processing |
The data were analyzed with Expression Console build 1.2.0.20, using Affymetrix default analysis settings and RMA+DABG (robust multichip average and detected above background) as normalization method.
|
|
|
Submission date |
Jun 19, 2013 |
Last update date |
Sep 13, 2014 |
Contact name |
Gloria Ribas |
E-mail(s) |
gribasdespuig@gmail.com
|
Organization name |
INCLIVA Biomedical Research Institute
|
Department |
Medical Oncology and Hematology Unit
|
Street address |
Blasco Ibañez, 17
|
City |
Valencia |
State/province |
Valencia |
ZIP/Postal code |
46010 |
Country |
Spain |
|
|
Platform ID |
GPL14613 |
Series (1) |
GSE48088 |
Expression of miRNAs in breast tumor from very youn women (<36) and women above 65 years old |
|