|
| Status |
Public on Sep 12, 2014 |
| Title |
BC_GR28 |
| Sample type |
RNA |
| |
|
| Source name |
Breast tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
subtype: Luminal/Her2+
age: 78
|
| Treatment protocol |
All samples did not receive neoadjuvant treatment
|
| Growth protocol |
All samples were locked in parafine-formaline (FFPE) and stored at room temperature
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from four 20μm-thick sections of FFPE tissue samples using RecoverAll Total Nucleic Acid Isolation Kit (Applied Biosystems, Life Technologies)
|
| Label |
biotin
|
| Label protocol |
300ng of total RNA was labeled with Hy3 fluorescent dye
|
| |
|
| Hybridization protocol |
RNA from each sample was hybridized over 18h at 56ºC onto a GeneChip miRNA 2.0 array
|
| Scan protocol |
Chips were scanned using an Agilent scanner, with the scan laser set to 635nm, scan resolution of 10μm, power 80 and PMT 70.
|
| Data processing |
The data were analyzed with Expression Console build 1.2.0.20, using Affymetrix default analysis settings and RMA+DABG (robust multichip average and detected above background) as normalization method.
|
| |
|
| Submission date |
Jun 19, 2013 |
| Last update date |
Sep 13, 2014 |
| Contact name |
Gloria Ribas |
| E-mail(s) |
gribasdespuig@gmail.com
|
| Organization name |
INCLIVA Biomedical Research Institute
|
| Department |
Medical Oncology and Hematology Unit
|
| Street address |
Blasco Ibañez, 17
|
| City |
Valencia |
| State/province |
Valencia |
| ZIP/Postal code |
46010 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE48088 |
Expression of miRNAs in breast tumor from very youn women (<36) and women above 65 years old |
|
