|
Status |
Public on Jul 16, 2013 |
Title |
genomic DNA from whole 2h sperm 1 |
Sample type |
genomic |
|
|
Source name |
sperm
|
Organism |
Homo sapiens |
Characteristics |
gender: male disease state: Normal donor
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Sperm DNA was extracted with an user-developed version of the QIAamp® DNeasy&Tissue Kit purification protocol according to manufacturer instructions.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
normal sperm sample EPI01_2h.20
|
Data processing |
Genome studio
|
|
|
Submission date |
Jun 04, 2013 |
Last update date |
Jul 16, 2013 |
Contact name |
Manel Esteller |
Organization name |
IDIBELL
|
Department |
PEBC
|
Lab |
Cancer Epigenetics
|
Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
City |
L'Hospitalet de Llobregat |
State/province |
Barcelona |
ZIP/Postal code |
08908 |
Country |
Spain |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE47627 |
Novel insights into DNA methylation features in spermatozoa: stability and peculiatities |
|