|
| Status |
Public on May 11, 2015 |
| Title |
Sw620 (HumanMethylation450 array) |
| Sample type |
genomic |
| |
|
| Source name |
Lymph node metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
tumor: lymph node metastasis
cell line: colon
|
| Growth protocol |
Cell were growth in DMEM complemented with FBS 10% and a combination of antibiotics and antimicotics
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extracted by Phenol:Chloroform:Isoamylalcohol (Sigma)
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Data processing |
Genome Studio V2011.2
|
| |
|
| Submission date |
Apr 26, 2013 |
| Last update date |
May 11, 2015 |
| Contact name |
Manel Esteller |
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE46419 |
A novel epigenetic regulated gene and involved in phenotypic switch classifies primary melanomas depending on the aggressiveness grade (Illumina) |
| GSE46522 |
A novel epigenetic regulated gene and involved in phenotypic switch classifies primary melanomas depending on the aggressiveness grade |
|
