|
| Status |
Public on Apr 24, 2013 |
| Title |
Patient 1, 1 month after start of IFN-beta therapy [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: clinically isolated syndrome
gender: female
age at ifn-beta therapy onset (baseline blood sampling): 28
duration from diagnosis to therapy initiation (in months): 1
edss at baseline: 1.0
edss after 1 year: 1.0
edss after 2 years: 1.0
number of relapses during the year prior to treatment: 1
number of relapses during 1-year follow-up: 0
number of relapses during 2-year follow-up: 0
completed years of ifn-beta therapy: 18
|
| Treatment protocol |
Patients were treated with subcutaneous IFN-beta-1b (Betaferon, Bayer HealthCare) at standard doses.
|
| Growth protocol |
Patient blood samples were taken immediately before first IFN-beta injection as well as one month post therapy initiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral blood mononuclear cells were separated using isopycnic centrifugation (Ficoll), and total RNA enriched with small RNAs was isolated using the mirVana miRNA isolation kit (Invitrogen) according to the manufacturers' protocols.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated RNA were prepared using the FlashTag Biotin HSR RNA labeling kit according to the Affymetrix protocol from 600 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Biotin-labeled RNA were hybridized for 16 h at 45°C on Affymetrix miRNA 2.0 arrays. The microarrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
microRNA expression data from a multiple sclerosis patient treated with IFN-beta
|
| Data processing |
The raw probe-level signals were converted to expression values using the RMA algorithm with quantile normalization. This resulted in expression levels for 20,706 probesets per chip.
|
| |
|
| Submission date |
Apr 22, 2013 |
| Last update date |
Jan 30, 2014 |
| Contact name |
Michael Hecker |
| E-mail(s) |
michael.hecker@rocketmail.com
|
| Organization name |
University of Rostock
|
| Department |
Department of Neurology
|
| Lab |
Division of Neuroimmunology
|
| Street address |
Gehlsheimer Str. 20
|
| City |
Rostock |
| ZIP/Postal code |
18147 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14613 |
| Series (2) |
| GSE46282 |
Expression data of multiple sclerosis patients receiving Interferon-beta therapy [miRNA-2_0] |
| GSE46293 |
Expression data of multiple sclerosis patients receiving Interferon-beta therapy |
|
