|
Status |
Public on Jul 01, 2013 |
Title |
6 Gy, 2 hr |
Sample type |
genomic |
|
|
Source name |
MDA-MB-231 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231 gender: female ionizing radiation treatment: 6 Gy, 2 hr disease state: breast cancer
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Qiagen EZ1 Advanced
|
Label |
Cy3 and Cy5
|
Label protocol |
The standard Infinium HD Methylation Assay protocol was used.
|
|
|
Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented, and hybridized to the Illumina Infinium HumanMethylation450 Beadchip using the standard Infinium HD Methylation Assay protocol.
|
Scan protocol |
Arrays were scanned on an Illumina iScan using Illumina recommended settings.
|
Description |
SZ-9
|
Data processing |
GenomeStudio v2011.1. Average Beta (Normalized using 'Controls' method; background subtracted). Unmethylated and methylated signal intensities (Normalized using 'Controls' method; background subtracted).
|
|
|
Submission date |
Apr 10, 2013 |
Last update date |
Jul 01, 2013 |
Contact name |
Steven P Zielske |
Organization name |
Wayne State University
|
Department |
Department of Radiation Oncology
|
Street address |
540 E. Canfield
|
City |
Detroit |
State/province |
MI |
ZIP/Postal code |
48201 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE45958 |
Radiation-induced epigenetic DNA methylation modification of radiation-response pathways |
|