|
| Status |
Public on Jan 01, 2016 |
| Title |
E-PZ-1-iPS-like-4 in control medium AR day 1 |
| Sample type |
genomic |
| |
|
| Source name |
E-PZ-1-iPS-like-4 in control medium AR day 1
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
cell line: E-PZ-1-iPS-like-4
cell type: induced pluripotent stem cells derived from prostate epithelial cells
disease state: normal
protocol: control medium AR
time: day 1
|
| Treatment protocol |
For prostate sphere differentiation: E-PZ-iPS-like cells cultured on Matrigel were digested with collagenase type IV (Invitrogen) and transferred to ultra-low attachment plates (Corning Life Sciences, Tewksbury, MA) for suspension culture for 8 days in DMEM/F12 (1:1) medium supplemented with 5 ng/ml bFGF (PeproTech), 10 ng/ml LIF (Santa Cruz Biotechnology Inc.), and 5% knockout FBS (Invitrogen). For basal epithelial cell differentiation, E-PZ-iPS-like spheres were then cultured in Complete PFMR-4A medium [29] for 3 days. To induce expression of androgen receptor (AR), E-PZ-iPS-like spheres were further cultured in Complete PFMR-4A medium with 10 nM R1881 for 3 days. To induce expression of prostate-specific antigen (PSA), E-PZ-iPS-like spheres were co-cultured with rat urogenital sinus (UGS) isolated from E17 rat embryos as previously described [31] in Complete PFMR-4A medium with 10 nM R1881 for 5 days.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from all samples using Qiagen AllPrep Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium HumanMethylation450K Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Sample name: AR day 1 control
normal iPS cells
|
| Data processing |
BeadStudio software v3.2
|
| |
|
| Submission date |
Mar 25, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Hongjuan Zhao |
| Organization name |
Stanford
|
| Department |
Urology
|
| Lab |
Donna Peehl
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
95014 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE45469 |
Induced Pluripotency of Human Prostatic Epithelial Cells |
|
