|
| Status |
Public on Apr 04, 2013 |
| Title |
VEH_mr4 |
| Sample type |
RNA |
| |
|
| Source name |
Oligodendroglioma
|
| Organism |
Homo sapiens |
| Characteristics |
mutation: R132H-IDH1
treatment: vehicle
tissue: Oligodendroglioma
|
| Treatment protocol |
Mice were treated with the indicated treated once a day by gavage.
|
| Growth protocol |
SCID mice were injected subcutaneously with 10^6 glioma cells, which were suspended in 100 mL of a 50:50 mixture of growth media and Matrigel (BD #356237). Once tumors had reached a measurable size, mice were randomized into the indicated treatment groups.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen RNeasy Lipid Tissue kit according to the manufactuer's protocol.
|
| Label |
biotin
|
| Label protocol |
200ng of your samples were amplified/labeled using the Affymetrix 3’IVT Express kit for 4 hours.
|
| |
|
| Hybridization protocol |
cRNA was hybridized according to standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
TS603 R132H Glioma Xenograft Experiment 1
Gene Expression Data from xenografts
|
| Data processing |
The data was analzyed using Partek Genomics Suite and Treeview software
|
| |
|
| Submission date |
Mar 15, 2013 |
| Last update date |
Apr 04, 2013 |
| Contact name |
Daniel Rohle |
| E-mail(s) |
rohled@mskcc.org
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
The Human Oncology and Pathogenesis Program
|
| Lab |
Ingo Mellinghoff Lab
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE45197 |
An Inhibitor of Mutant IDH1 Delays Growth and Promotes Differentiation of Glioma Cells Expression data for Xenograft Samples |
| GSE45200 |
An Inhibitor of Mutant IDH1 Delays Growth and Promotes Differentiation of Glioma Cells |
|
