|
Status |
Public on Apr 04, 2013 |
Title |
IDH1i_975 |
Sample type |
RNA |
|
|
Source name |
Oligodendroglioma
|
Organism |
Homo sapiens |
Characteristics |
mutation: R132H-IDH1 treatment: AGI-5198 tissue: Oligodendroglioma
|
Treatment protocol |
Mice were treated with the indicated treated once a day by gavage.
|
Growth protocol |
SCID mice were injected subcutaneously with 10^6 glioma cells, which were suspended in 100 mL of a 50:50 mixture of growth media and Matrigel (BD #356237). Once tumors had reached a measurable size, mice were randomized into the indicated treatment groups.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Qiagen RNeasy Lipid Tissue kit according to the manufactuer's protocol.
|
Label |
biotin
|
Label protocol |
200ng of your samples were amplified/labeled using the Affymetrix 3’IVT Express kit for 4 hours.
|
|
|
Hybridization protocol |
cRNA was hybridized according to standard Affymetrix protocol.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
TS603 R132H Glioma Xenograft Experiment 1 Gene Expression Data from xenografts
|
Data processing |
The data was analzyed using Partek Genomics Suite and Treeview software
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|
|
Submission date |
Mar 15, 2013 |
Last update date |
Apr 04, 2013 |
Contact name |
Daniel Rohle |
E-mail(s) |
rohled@mskcc.org
|
Organization name |
Memorial Sloan Kettering Cancer Center
|
Department |
The Human Oncology and Pathogenesis Program
|
Lab |
Ingo Mellinghoff Lab
|
Street address |
1275 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE45197 |
An Inhibitor of Mutant IDH1 Delays Growth and Promotes Differentiation of Glioma Cells Expression data for Xenograft Samples |
GSE45200 |
An Inhibitor of Mutant IDH1 Delays Growth and Promotes Differentiation of Glioma Cells |
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