|
| Status |
Public on Jun 12, 2013 |
| Title |
PA_52 |
| Sample type |
genomic |
| |
|
| Source name |
Frozen pilocytic astrocytoma tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pilocytic astrocytoma tumor
brain location: Cerebellum
age at diagnosis: 9 y
gender: Male
dna methylation subgroup: 1
mapk hit: KIAA1549:BRAF fusion_16:9
|
| Treatment protocol |
Tumor samples were snap frozen upon resection and stored at -80 oC until DNA extraction. Tumor content was assessed from equivalent material fixed in formalin and used for histological diagnosis (all samples contained ≥70% tumor cells).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
High molecular weight DNA was extracted by use of a salting-out method or by ultracentrifugation over cesium chloride
|
| Label |
Biotin/DNP
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
standard Illumina protocol
|
| Scan protocol |
Illumina iScan System according to the manufacturer's protocol.
|
| Description |
Pilocytic astrocytoma, Cerebellum, 9, Male, 1, KIAA1549:BRAF fusion_16:9
|
| Data processing |
Data analysis was performed using GenomeStudio 2011.1 (methylationmodule 1.9.0) and anootation 1.2. The methylation analysis was performed as recommended by Illumina, with no background subtraction and normalisation to internal controls.
|
| |
|
| Submission date |
Feb 26, 2013 |
| Last update date |
Jun 12, 2013 |
| Contact name |
Sally R Lambert |
| E-mail(s) |
sally_lambert40@hotmail.com
|
| Organization name |
Queen Mary, University of London
|
| Department |
Centre for Cutaneous Research
|
| Street address |
Blizard Institute, 4 Newark Street
|
| City |
London |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE44684 |
DNA methylation data from pilocytic astrocytoma tumor samples and normal cerebellum controls |
|
